Persistent organic pollutants contamination is concered because it is hard to biodegrade. Understanding the structure of the soil microbial communities and the metabolic pathway of POPs for the bioremediation of contaminated soil is extremely important. We apply next-generation sequencing technology for analysis of microorganism diversity changes and transcriptomics of biodegraded bacteria in polychlorinated biphenyls or pentachlorophenol contaminated soil. It was found that would make the three-fold increase Proteobacteria after pentachlorophenol contamination. PCB contamination will cause an increase of 1.3 times in Proteobacteria and 1.4 times in Actinobacteria. The microorganism community is still different between two soils even when they undergo the same pollutant by the beta diversity and heat map analysis. The specifity of microorganism community can further applied in environmental forensics. Analysis of two PCBs degraded bacteria and a PCP degraded bacteria, though not directly related to metabolic genes found, but there are still a number of transcricpts with very different expression can be used as candidate biomarkers.

Next-generation sequencing, Polychlorinated biphenyls, Pentachlorophenol